› 3kzh

X-ray diffraction
2.45Å resolution

Crystal structure of a putative sugar kinase from Clostridium perfringens

Released:
DOI: 10.2210/pdb3kzh/pdb
PDB entry 3kzh coloured by chain, front view.
PDB entry 3kzh coloured by chain, side view.
PDB entry 3kzh coloured by chain, top view.
Source organism: Clostridium perfringens
Entry authors: Syed Ibrahim B, Kumaran D, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186894 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate kinase PfkB domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 37.2 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8XKB8 (Residues: 3-320; Coverage: 99%)
Gene name: CPE1482
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

1 bound ligand:
Ligand BGC 2 x BGC
1 modified residue:
Ligand MSE 26 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 52.396Å b: 108.102Å c: 110.918Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.231 0.273
Expression system: Escherichia coli BL21(DE3)

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