› 3l0j

X-ray diffraction
2.4Å resolution

Crystal structure of orphan nuclear receptor RORgamma in complex with natural ligand

Released:
DOI: 10.2210/pdb3l0j/pdb
PDB entry 3l0j coloured by chain, front view.
PDB entry 3l0j coloured by chain, side view.
PDB entry 3l0j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for hydroxycholesterols as natural ligands of orphan nuclear receptor RORgamma.
Jin L, Martynowski D, Zheng S, Wada T, Xie W, Li Y
Mol Endocrinol 24 923-9 (2010)
PMID: 20203100

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156333 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclear receptor ROR-gamma Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 28.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P51449 (Residues: 265-507; Coverage: 47%)
Gene names: NR1F3, RORC, RORG, RZRG
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15596 (Residues: 688-697; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

1 bound ligand:
Ligand HC9 1 x HC9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P41212
Unit cell:
a: 58.502Å b: 58.502Å c: 159.032Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.193 0.283
Expression system: Escherichia coli

Quick links

Citations

39 review citations

25-Hydroxycholesterols in innate and adaptive immunity.
Cyster et al. (2014)
38 more

11 mentions without citation

ROR nuclear receptors: structures, related diseases, and drug discovery.
Zhang et al. (2015)
10 more