› 3l2j

X-ray diffraction
3.24Å resolution

Dimeric structure of the ligand-free extracellular domain of the human parathyroid hormone receptor (PTH1R)

Released:
DOI: 10.2210/pdb3l2j/pdb
PDB entry 3l2j coloured by chain, front view.
PDB entry 3l2j coloured by chain, side view.
PDB entry 3l2j coloured by chain, top view.
Source organisms:
Primary publication:
Dimeric arrangement of the parathyroid hormone receptor and a structural mechanism for ligand-induced dissociation.
Pioszak AA, Harikumar KG, Parker NR, Miller LJ, Xu HE
J Biol Chem 285 12435-44 (2010)
PMID: 20172855

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Parathyroid hormone/parathyroid hormone-related peptide receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 535 amino acids
Theoretical weight: 59.85 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 29-392; Coverage: 98%)
  • Canonical: Q03431 (Residues: 29-187; Coverage: 28%)
Gene names: JW3994, PTH1R, PTHR, PTHR1, b4034, malE
Sequence domains:
Structure domains: GPCR, family 2, extracellular hormone receptor domain

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2221
Unit cell:
a: 136.702Å b: 182.326Å c: 97.166Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.225 0.258
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Insights into the structure of class B GPCRs.
Hollenstein et al. (2014)
6 more

5 mentions without citation

The significance of G protein-coupled receptor crystallography for drug discovery.
Salon et al. (2011)
4 more