› 3l44

X-ray diffraction
2.05Å resolution

Crystal structure of Bacillus anthracis HemL-1, glutamate semialdehyde aminotransferase

Released:
DOI: 10.2210/pdb3l44/pdb
PDB entry 3l44 coloured by chain, front view.
PDB entry 3l44 coloured by chain, side view.
PDB entry 3l44 coloured by chain, top view.
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Entry authors: Anderson SM, Wawrzak Z, Gordon E, Hasseman J, Edwards A, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(S)-4-amino-5-oxopentanoate = 5-aminolevulinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate-1-semialdehyde 2,1-aminomutase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 434 amino acids
Theoretical weight: 47.24 KDa
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q81YV0 (Residues: 1-434; Coverage: 100%)
Gene names: BAS0499, BA_0531, GBAA_0531, hemL1
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 24 x MSE
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 75.84Å b: 93.92Å c: 105.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.157 0.192
Expression system: Escherichia coli BL21(DE3)

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