› 3l4h

X-ray diffraction
1.8Å resolution

Helical box domain and second WW domain of the human E3 ubiquitin-protein ligase HECW1

Released:
DOI: 10.2210/pdb3l4h/pdb
PDB entry 3l4h coloured by chain, front view.
PDB entry 3l4h coloured by chain, side view.
PDB entry 3l4h coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Walker JR, Qiu L, Li Y, Weigelt J, Bountra C, Arrowsmith CH, Edwards AM, Botchkarev A, Dhe-Paganon S, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181185 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase HECW1 Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 12.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q76N89 (Residues: 948-1056; Coverage: 7%)
Gene names: HECW1, KIAA0322, NEDL1
Sequence domains: Helical box domain of E3 ubiquitin-protein ligase HECW1

Ligands and Environments

1 bound ligand:
Ligand ACY 1 x ACY
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 26.079Å b: 44.082Å c: 39.61Å
α: 90° β: 92.54° γ: 90°
R-values:
R R work R free
0.152 0.15 0.2
Expression system: Escherichia coli

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