› 3l6e

X-ray diffraction
2.3Å resolution

Crystal structure of putative short chain dehydrogenase/reductase family oxidoreductase from Aeromonas hydrophila subsp. hydrophila ATCC 7966

Released:
DOI: 10.2210/pdb3l6e/pdb
PDB entry 3l6e coloured by chain, front view.
PDB entry 3l6e coloured by chain, side view.
PDB entry 3l6e coloured by chain, top view.
Source organism: Aeromonas hydrophila subsp. hydrophila ATCC 7966
Entry authors: Malashkevich VN, Patskovsky Y, Toro R, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Sphinganine + NADP(+) = 3-dehydrosphinganine + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-106112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-dehydrosphinganine reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 25.81 KDa
Source organism: Aeromonas hydrophila subsp. hydrophila ATCC 7966
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0KPA6 (Residues: 2-225; Coverage: 100%)
Gene name: AHA_3651
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 127.363Å b: 53.005Å c: 67.019Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.27
Expression system: Escherichia coli BL21(DE3)

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