› 3l9e

X-ray diffraction
2.05Å resolution

Crystal structures of holo and Cu-deficient Cu/ZnSOD from the silkworm Bombyx mori and the implications in Amyotrophic lateral sclerosis

Released:
DOI: 10.2210/pdb3l9e/pdb
PDB entry 3l9e coloured by chain, front view.
PDB entry 3l9e coloured by chain, side view.
PDB entry 3l9e coloured by chain, top view.
Source organism: Bombyx mori
Primary publication:
Crystal structures of holo and Cu-deficient Cu/Zn-SOD from the silkworm Bombyx mori and the implications in amyotrophic lateral sclerosis.
Zhang NN, He YX, Li WF, Teng YB, Yu J, Chen Y, Zhou CZ
Proteins 78 1999-2004 (2010)
PMID: 20310068

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160610 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Cu-Zn] Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 154 amino acids
Theoretical weight: 15.86 KDa
Source organism: Bombyx mori
Expression system: Escherichia coli
UniProt:
  • Canonical: P82205 (Residues: 1-154; Coverage: 100%)
Sequence domains: Copper/zinc superoxide dismutase (SODC)
Structure domains: Superoxide dismutase, copper/zinc binding domain

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P65
Unit cell:
a: 118.47Å b: 118.47Å c: 81.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.248
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
5 other articles

1 mention without citation

Characteristics of the Copper,Zinc Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench.
Li et al. (2018)