› 3l9i

X-ray diffraction
2.2Å resolution

Myosin VI nucleotide-free (mdinsert2) L310G mutant crystal structure

Released:
DOI: 10.2210/pdb3l9i/pdb
PDB entry 3l9i coloured by chain, front view.
PDB entry 3l9i coloured by chain, side view.
PDB entry 3l9i coloured by chain, top view.
Source organisms:
Entry authors: Pylypenko O, Song L, Sweeney LH, Houdusse A

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158578 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Unconventional myosin-VI Chain: A
Molecule details ›
Chain: A
Length: 814 amino acids
Theoretical weight: 92.98 KDa
Source organism: Sus scrofa
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q29122 (Residues: 2-816; Coverage: 65%)
Gene name: MYO6
Sequence domains: Myosin head (motor domain)
Calmodulin Chain: C
Molecule details ›
Chain: C
Length: 149 amino acids
Theoretical weight: 16.83 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62152 (Residues: 1-149; Coverage: 100%)
Gene names: CG8472, Cam
Sequence domains: EF-hand domain pair

Ligands and Environments

4 bound ligands:
Ligand TBU 1 x TBU
Ligand ACT 2 x ACT
Ligand PO4 4 x PO4
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 68.54Å b: 104.4Å c: 90.33Å
α: 90° β: 91.09° γ: 90°
R-values:
R R work R free
0.187 0.185 0.235
Expression system: Spodoptera frugiperda

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