› 3lao

X-ray diffraction
2.4Å resolution

Crystal Structure of Enoyl-CoA Hydratase from Pseudomonas aeruginosa PA01

Released:
DOI: 10.2210/pdb3lao/pdb
PDB entry 3lao coloured by chain, front view.
PDB entry 3lao coloured by chain, side view.
PDB entry 3lao coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Kim Y, Chhor G, Buck K, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191358 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable enoyl-CoA hydratase/isomerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 258 amino acids
Theoretical weight: 28.14 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9I498 (Residues: 1-255; Coverage: 96%)
Gene name: PA1240
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains: 2-enoyl-CoA Hydratase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand GOL 7 x GOL
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 49.362Å b: 101.616Å c: 141.536Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.172 0.239
Expression system: Escherichia coli BL21

Quick links