› 3laq

X-ray diffraction
3.2Å resolution

Structure-based engineering of species selectivity in the uPA-uPAR interaction

Released:
DOI: 10.2210/pdb3laq/pdb
PDB entry 3laq coloured by chain, front view.
PDB entry 3laq coloured by chain, side view.
PDB entry 3laq coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure-based engineering of species selectivity in the interaction between urokinase and its receptor: implication for preclinical cancer therapy.
Lin L, Gårdsvoll H, Huai Q, Huang M, Ploug M
J Biol Chem 285 10982-92 (2010)
PMID: 20133942

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139267 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Urokinase-type plasminogen activator long chain A Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 15 KDa
Source organism: Mus musculus
Expression system: Drosophila
UniProt:
  • Canonical: P06869 (Residues: 21-154; Coverage: 32%)
Gene name: Plau
Sequence domains: Kringle domain
Structure domains:
Urokinase plasminogen activator surface receptor Chains: U, V
Molecule details ›
Chains: U, V
Length: 277 amino acids
Theoretical weight: 30.17 KDa
Source organism: Mus musculus
Expression system: Drosophila
UniProt:
  • Canonical: P35456 (Residues: 24-300; Coverage: 91%)
Gene name: Plaur
Sequence domains: u-PAR/Ly-6 domain
Structure domains: CD59

Ligands and Environments

1 bound ligand:
Ligand NAG 10 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 61.421Å b: 137.248Å c: 65.785Å
α: 90° β: 106.17° γ: 90°
R-values:
R R work R free
0.237 0.231 0.341
Expression system: Drosophila

Quick links

Citations

10 review citations

GPIHBP1, an endothelial cell transporter for lipoprotein lipase.
Young et al. (2011)
9 more

2 mentions without citation

Structure-based engineering of species selectivity in the interaction between urokinase and its receptor: implication for preclinical cancer therapy.
Lin et al. (2010)
1 more