› 3law

X-ray diffraction
2.8Å resolution

Structure of GTP-bound L129F mutant Rab7

Released:
DOI: 10.2210/pdb3law/pdb
PDB entry 3law coloured by chain, front view.
PDB entry 3law coloured by chain, side view.
PDB entry 3law coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.
McCray BA, Skordalakes E, Taylor JP
Hum Mol Genet 19 1033-47 (2010)
PMID: 20028791

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-7a Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 207 amino acids
Theoretical weight: 23.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P51149 (Residues: 1-207; Coverage: 100%)
Gene names: RAB7, RAB7A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 5 x MG
Ligand GNP 5 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P1
Unit cell:
a: 35.554Å b: 89.335Å c: 89.378Å
α: 71.24° β: 85.97° γ: 85.91°
R-values:
R R work R free
0.237 0.22 0.273
Expression system: Escherichia coli

Quick links

Citations

31 review citations

The role of autophagy in neurodegenerative disease.
Nixon RA. (2013)
30 more

3 mentions without citation

In silico identification of AMPylating enzymes and study of their divergent evolution.
Khater et al. (2015)
2 more