› 3led

X-ray diffraction
1.45Å resolution

Crystal structure of 3-oxoacyl-(acyl carrier protein) synthase III from Rhodopseudomonas palustris CGA009

Released:
DOI: 10.2210/pdb3led/pdb
PDB entry 3led coloured by chain, front view.
PDB entry 3led coloured by chain, side view.
PDB entry 3led coloured by chain, top view.
Source organism: Rhodopseudomonas palustris CGA009
Entry authors: Chang C, Xu X, Cui H, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179992 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-ketoacyl-ACP synthase III Chains: A, B
Molecule details ›
Chains: A, B
Length: 392 amino acids
Theoretical weight: 43.23 KDa
Source organism: Rhodopseudomonas palustris CGA009
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6NA87 (Residues: 2-371; Coverage: 100%)
Gene names: RPA1298, TX73_006660
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand FMT 2 x FMT
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 111.997Å b: 114.79Å c: 60.429Å
α: 90° β: 96.8° γ: 90°
R-values:
R R work R free
0.146 0.145 0.168
Expression system: Escherichia coli BL21(DE3)

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