› 3leq

X-ray diffraction
1.85Å resolution

The Crystal Structure of the Roadblock/LC7 domain from Streptomyces avermitillis to 1.85A

Released:
DOI: 10.2210/pdb3leq/pdb
PDB entry 3leq coloured by chain, front view.
PDB entry 3leq coloured by chain, side view.
PDB entry 3leq coloured by chain, top view.
Source organism: Streptomyces avermitilis MA-4680 = NBRC 14893
Entry authors: Stein AJ, Xu X, Cui H, Ng J, Edwards A, Savchenko A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-182665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Roadblock/LAMTOR2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 13.56 KDa
Source organism: Streptomyces avermitilis MA-4680 = NBRC 14893
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82L23 (Residues: 8-133; Coverage: 88%)
Gene names: SAVERM_2189, cvnB5
Sequence domains: Roadblock/LC7 domain
Structure domains: Dynein light chain 2a, cytoplasmic

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 89.466Å b: 29.824Å c: 40.752Å
α: 90° β: 104.89° γ: 90°
R-values:
R R work R free
0.217 0.216 0.236
Expression system: Escherichia coli BL21(DE3)

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