› 3ler

X-ray diffraction
1.84Å resolution

Crystal Structure of Dihydrodipicolinate Synthase from Campylobacter jejuni subsp. jejuni NCTC 11168

Released:
DOI: 10.2210/pdb3ler/pdb
PDB entry 3ler coloured by chain, front view.
PDB entry 3ler coloured by chain, side view.
PDB entry 3ler coloured by chain, top view.
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Entry authors: Kim Y, Zhou M, Hasseman J, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192936 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-tetrahydrodipicolinate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 301 amino acids
Theoretical weight: 33.38 KDa
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9PPB4 (Residues: 1-298; Coverage: 100%)
Gene names: Cj0806, dapA
Sequence domains: Dihydrodipicolinate synthetase family
Structure domains: Aldolase class I

Ligands and Environments

5 bound ligands:
Ligand EDO 2 x EDO
Ligand MG 1 x MG
Ligand FMT 1 x FMT
Ligand PEG 1 x PEG
Ligand ACY 1 x ACY
2 modified residues:
Ligand MSE 28 x MSE
Ligand KPI 4 x KPI

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 72.185Å b: 85.304Å c: 199.157Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.19
Expression system: Escherichia coli

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