› 3lf3

X-ray diffraction
1.15Å resolution

Crystal Structure of Fast Fluorescent Timer Fast-FT

Released:
DOI: 10.2210/pdb3lf3/pdb
Model geometry
Fit model/data
PDB entry 3lf3 coloured by chain, front view.
PDB entry 3lf3 coloured by chain, side view.
PDB entry 3lf3 coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
Understanding blue-to-red conversion in monomeric fluorescent timers and hydrolytic degradation of their chromophores.
Pletnev S, Subach FV, Dauter Z, Wlodawer A, Verkhusha VV
J Am Chem Soc 132 2243-53 (2010)
PMID: 20121102

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112322 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 26.33 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: D1MPT3 (Residues: 6-236; Coverage: 98%)
Gene names: PAmCherry, PAmCherry1
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
2 modified residues:
Ligand NFA 1 x NFA
Ligand NRQ 1 x NRQ

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 48.838Å b: 42.517Å c: 61.766Å
α: 90° β: 112.07° γ: 90°
R-values:
R R work R free
0.15 0.149 0.177
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells.
Newman et al. (2011)
7 more

2 mentions without citation

Hydroxyethylamine Based Phthalimides as New Class of Plasmepsin Hits: Design, Synthesis and Antimalarial Evaluation.
Singh et al. (2015)
1 more