› 3lgr

X-ray diffraction
1.64Å resolution

Xylanase II from Trichoderma reesei cocrystallized with tris-dipicolinate europium

Released:
DOI: 10.2210/pdb3lgr/pdb
PDB entry 3lgr coloured by chain, front view.
PDB entry 3lgr coloured by chain, side view.
PDB entry 3lgr coloured by chain, top view.
Source organism: Trichoderma reesei
Primary publication:
A dipicolinate lanthanide complex for solving protein structures using anomalous diffraction.
Pompidor G, Maury O, Vicat J, Kahn R
Acta Crystallogr D Biol Crystallogr 66 762-9 (2010)
PMID: 20606256

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase 2 Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 20.84 KDa
Source organism: Trichoderma reesei
Expression system: Trichoderma longibrachiatum
UniProt:
  • Canonical: P36217 (Residues: 35-223; Coverage: 93%)
Gene names: M419DRAFT_124931, xyn2
Sequence domains: Glycosyl hydrolases family 11
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand EU 3 x EU
Ligand PDC 3 x PDC
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 110.89Å b: 38.37Å c: 47.59Å
α: 90° β: 99.83° γ: 90°
R-values:
R R work R free
0.139 0.138 0.168
Expression system: Trichoderma longibrachiatum

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Citations

11 citation in other articles

Crystal structure of pb9, the distal tail protein of bacteriophage T5: a conserved structural motif among all siphophages.
Flayhan et al. (2014)
10 more