› 3lib

X-ray diffraction
2.99Å resolution

Crystal Structure of the extracellular domain of the putative histidine kinase mmHK1S-Z3

Released:
DOI: 10.2210/pdb3lib/pdb
PDB entry 3lib coloured by chain, front view.
PDB entry 3lib coloured by chain, side view.
PDB entry 3lib coloured by chain, top view.
Source organism: Methanosarcina mazei
Primary publication:
Structural characterization of the predominant family of histidine kinase sensor domains.
Zhang Z, Hendrickson WA
J Mol Biol 400 335-53 (2010)
PMID: 20435045

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-185797 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 290 amino acids
Theoretical weight: 32.48 KDa
Source organism: Methanosarcina mazei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8PSW1 (Residues: 32-312; Coverage: 34%)
Gene name: MM_2965
Sequence domains: Cache domain
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand K 10 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P65
Unit cell:
a: 129.524Å b: 129.524Å c: 404.729Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.197 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Evolution of two-component signal transduction systems.
Capra et al. (2012)
18 more

11 mentions without citation

Diversity in Sensing and Signaling of Bacterial Sensor Histidine Kinases.
Ishii et al. (2021)
10 more