› 3lic

X-ray diffraction
2.3Å resolution

Crystal Structure of the extracellular domain of the putative histidine kinase soHK1S-Z6

Released:
DOI: 10.2210/pdb3lic/pdb
PDB entry 3lic coloured by chain, front view.
PDB entry 3lic coloured by chain, side view.
PDB entry 3lic coloured by chain, top view.
Source organism: Shewanella oneidensis
Primary publication:
Structural characterization of the predominant family of histidine kinase sensor domains.
Zhang Z, Hendrickson WA
J Mol Biol 400 335-53 (2010)
PMID: 20435045

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-184407 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 30.64 KDa
Source organism: Shewanella oneidensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EII0 (Residues: 36-308; Coverage: 16%)
Gene name: SO_0859
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P3121
Unit cell:
a: 133.069Å b: 133.069Å c: 32.369Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.181 0.245
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Evolution of two-component signal transduction systems.
Capra et al. (2012)
18 more

9 mentions without citation

Diversity in Sensing and Signaling of Bacterial Sensor Histidine Kinases.
Ishii et al. (2021)
8 more