› 3ljf

X-ray diffraction
2.1Å resolution

The X-ray structure of iron superoxide dismutase from Pseudoalteromonas haloplanktis (crystal form II)

Released:
DOI: 10.2210/pdb3ljf/pdb
PDB entry 3ljf coloured by chain, front view.
PDB entry 3ljf coloured by chain, side view.
PDB entry 3ljf coloured by chain, top view.
Source organism: Pseudoalteromonas translucida TAC125
Primary publication:
Structure and flexibility in cold-adapted iron superoxide dismutases: the case of the enzyme isolated from Pseudoalteromonas haloplanktis.
Merlino A, Russo Krauss I, Castellano I, De Vendittis E, Rossi B, Conte M, Vergara A, Sica F
J Struct Biol 172 343-52 (2010)
PMID: 20732427

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160896 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Superoxide dismutase [Fe] Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 192 amino acids
Theoretical weight: 21.27 KDa
Source organism: Pseudoalteromonas translucida TAC125
Expression system: Escherichia coli
UniProt:
  • Canonical: P84612 (Residues: 1-192; Coverage: 100%)
Gene names: PSHAa1215, sodB
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand FE 4 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 50.476Å b: 103.785Å c: 90.251Å
α: 90° β: 103.81° γ: 90°
R-values:
R R work R free
0.215 0.192 0.248
Expression system: Escherichia coli

Quick links

Citations

8 review citations

tRNA Modifications: Impact on Structure and Thermal Adaptation.
Lorenz et al. (2017)
7 more

2 mentions without citation

Psychrophilic enzymes: from folding to function and biotechnology.
Feller G. (2013)
1 more