3lku

X-ray diffraction
2.8Å resolution

Crystal structure of S. cerevisiae Get4 in complex with an N-terminal fragment of Get5

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural characterization of the Get4/Get5 complex and its interaction with Get3.
Proc Natl Acad Sci U S A 107 12127-32 (2010)
PMID: 20554915

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-171300 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Golgi to ER traffic protein 4 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 292 amino acids
Theoretical weight: 34.43 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12125 (Residues: 11-300; Coverage: 93%)
Gene names: GET4, O3580, YOR164C
Sequence domains: Golgi to ER traffic protein 4
Structure domains: Tetratricopeptide repeat domain
Ubiquitin-like protein MDY2 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 54 amino acids
Theoretical weight: 6.09 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12285 (Residues: 3-56; Coverage: 26%)
Gene names: GET5, MDY2, TMA24, YOL111C
Sequence domains: Binding domain to Get4 on Get5, Golgi to ER traffic protein
Structure domains: Single helix bin

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P3121
Unit cell:
a: 108.733Å b: 108.733Å c: 169.811Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.226
Expression system: Escherichia coli