› 3ll2

X-ray diffraction
0.97Å resolution

Monomeric Griffithsin in Complex with a High-Mannose Branched Carbohydrate

Released:
DOI: 10.2210/pdb3ll2/pdb
PDB entry 3ll2 coloured by chain, front view.
PDB entry 3ll2 coloured by chain, side view.
PDB entry 3ll2 coloured by chain, top view.
Source organism: Griffithsia sp. Q66D336
Primary publication:
Monomerization of viral entry inhibitor griffithsin elucidates the relationship between multivalent binding to carbohydrates and anti-HIV activity.
Moulaei T, Shenoy SR, Giomarelli B, Thomas C, McMahon JB, Dauter Z, O'Keefe BR, Wlodawer A
Structure 18 1104-15 (2010)
PMID: 20826337

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160912 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Griffithsin Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 12.82 KDa
Source organism: Griffithsia sp. Q66D336
Expression system: Escherichia coli
UniProt:
  • Canonical: P84801 (Residues: 1-16, 17-121; Coverage: 100%)
Sequence domains: Jacalin-like lectin domain
Structure domains: Jacalin-like lectin domain

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
2 bound ligands:
Ligand EDO 7 x EDO
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I41
Unit cell:
a: 90.5Å b: 90.5Å c: 27.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.143 0.155
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Antiviral drug discovery: broad-spectrum drugs from nature.
Martinez et al. (2015)
11 more

13 mentions without citation

Potential Applications of Plant Biotechnology against SARS-CoV-2.
Capell et al. (2020)
12 more