› 3lny

X-ray diffraction
1.3Å resolution

Second PDZ domain from human PTP1E in complex with RA-GEF2 peptide

Released:
DOI: 10.2210/pdb3lny/pdb
PDB entry 3lny coloured by chain, front view.
PDB entry 3lny coloured by chain, side view.
PDB entry 3lny coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallographic and nuclear magnetic resonance evaluation of the impact of peptide binding to the second PDZ domain of protein tyrosine phosphatase 1E.
Zhang J, Sapienza PJ, Ke H, Chang A, Hengel SR, Wang H, Phillips GN, Lee AL
Biochemistry 49 9280-91 (2010)
PMID: 20839809

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171441 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 13 Chain: A
Molecule details ›
Chain: A
Length: 96 amino acids
Theoretical weight: 10.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12923 (Residues: 1361-1456; Coverage: 4%)
Gene names: PNP1, PTP1E, PTPL1, PTPN13
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain
Rap guanine nucleotide exchange factor 6 Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 632 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q8TEU7 (Residues: 1596-1601; Coverage: 0%)
Gene names: PDZGEF2, RAPGEF6

Ligands and Environments

2 bound ligands:
Ligand SCN 1 x SCN
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: R32
Unit cell:
a: 73.965Å b: 73.965Å c: 134.056Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.163 0.189
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

Dynamic allostery: linkers are not merely flexible.
Ma et al. (2011)
7 more

20 mentions without citation

Crystallographic and nuclear magnetic resonance evaluation of the impact of peptide binding to the second PDZ domain of protein tyrosine phosphatase 1E.
Zhang et al. (2010)
19 more