› 3lop

X-ray diffraction
1.55Å resolution

Crystal structure of substrate-binding periplasmic protein (Pbp) from Ralstonia solanacearum

Released:
DOI: 10.2210/pdb3lop/pdb
PDB entry 3lop coloured by chain, front view.
PDB entry 3lop coloured by chain, side view.
PDB entry 3lop coloured by chain, top view.
Source organism: Ralstonia solanacearum GMI1000
Entry authors: Palani K, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186915 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine-binding protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 39.22 KDa
Source organism: Ralstonia solanacearum GMI1000
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8XUX2 (Residues: 33-388; Coverage: 100%)
Gene name: RSc3061
Sequence domains: Periplasmic binding protein
Structure domains: Rossmann fold

Ligands and Environments

5 bound ligands:
Ligand LEU 1 x LEU
Ligand EDO 5 x EDO
Ligand NI 2 x NI
Ligand MG 1 x MG
Ligand MN 1 x MN
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 44.11Å b: 64.739Å c: 114.085Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.179 0.195
Expression system: Escherichia coli BL21(DE3)

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