› 3lrq

X-ray diffraction
2.29Å resolution

Crystal structure of the U-box domain of human ubiquitin-protein ligase (E3), NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET HR4604D.

Released:
DOI: 10.2210/pdb3lrq/pdb
PDB entry 3lrq coloured by chain, front view.
PDB entry 3lrq coloured by chain, side view.
PDB entry 3lrq coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kuzin A, Chen Y, Seetharaman J, Mao M, Xiao R, Ciccosanti C, Shastry R, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131805 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase TRIM37 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 100 amino acids
Theoretical weight: 12.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O94972 (Residues: 1-90; Coverage: 9%)
Gene names: KIAA0898, MUL, POB1, TRIM37
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21
Unit cell:
a: 72.544Å b: 34.922Å c: 86.709Å
α: 90° β: 90.78° γ: 90°
R-values:
R R work R free
0.192 0.19 0.245
Expression system: Escherichia coli BL21(DE3)

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