› 3lyq

X-ray diffraction
2.3Å resolution

Crystal structure of IpgB2 from Shigella flexneri

Released:
DOI: 10.2210/pdb3lyq/pdb
PDB entry 3lyq coloured by chain, front view.
PDB entry 3lyq coloured by chain, side view.
PDB entry 3lyq coloured by chain, top view.
Source organism: Shigella flexneri
Primary publication:
Structure of Shigella IpgB2 in complex with human RhoA: implications for the mechanism of bacterial guanine nucleotide exchange factor mimicry.
Klink BU, Barden S, Heidler TV, Borchers C, Ladwein M, Stradal TE, Rottner K, Heinz DW
J Biol Chem 285 17197-208 (2010)
PMID: 20363740

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
IpgB2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 21.94 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9AJW7 (Residues: 1-188; Coverage: 100%)
Gene names: SFLP025, SF_p0024, ipgB2
Sequence domains: IpaB/EvcA family
Structure domains: SopE-like GEF fold

Ligands and Environments

2 bound ligands:
Ligand FLC 3 x FLC
Ligand FEO 2 x FEO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P4212
Unit cell:
a: 114.08Å b: 114.08Å c: 88.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.229 0.292
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
14 more

3 mentions without citation

Mimicking GEFs: a common theme for bacterial pathogens.
Orchard et al. (2012)
2 more