› 3lyu

X-ray diffraction
2.3Å resolution

Crystal Structure of the C-terminal domain (residues 83-215) of PF1911 hydrogenase from Pyrococcus furiosus, Northeast Structural Genomics Consortium Target PfR246A

Released:
DOI: 10.2210/pdb3lyu/pdb
PDB entry 3lyu coloured by chain, front view.
PDB entry 3lyu coloured by chain, side view.
PDB entry 3lyu coloured by chain, top view.
Source organism: Pyrococcus furiosus
Entry authors: Forouhar F, Abashidze M, Seetharaman J, Sahdev S, Xiao R, Foote EL, Ciccosanti C, Belote RL, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
homo hexamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186344 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding FR-type domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 142 amino acids
Theoretical weight: 16.41 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TZS3 (Residues: 83-215; Coverage: 46%)
Gene name: PF1911
Structure domains: Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P31
Unit cell:
a: 77.654Å b: 77.654Å c: 117.513Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.21 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links