› 3m1n

X-ray diffraction
1.85Å resolution

Crystal structure of Human Sonic Hedgehog N-terminal domain

Released:
DOI: 10.2210/pdb3m1n/pdb
PDB entry 3m1n coloured by chain, front view.
PDB entry 3m1n coloured by chain, side view.
PDB entry 3m1n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mapping sonic hedgehog-receptor interactions by steric interference.
Pepinsky RB, Rayhorn P, Day ES, Dergay A, Williams KP, Galdes A, Taylor FR, Boriack-Sjodin PA, Garber EA
J Biol Chem 275 10995-1001 (2000)
PMID: 10753901

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sonic hedgehog protein N-product Chains: A, B
Molecule details ›
Chains: A, B
Length: 175 amino acids
Theoretical weight: 19.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15465 (Residues: 23-197; Coverage: 40%)
Gene name: SHH
Sequence domains: Hedgehog amino-terminal signalling domain
Structure domains: Muramoyl-pentapeptide Carboxypeptidase; domain 2

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 45.741Å b: 51.788Å c: 69.932Å
α: 90° β: 96.53° γ: 90°
R-values:
R R work R free
0.206 0.203 0.26
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Cancer metastasis facilitated by developmental pathways: Sonic hedgehog, Notch, and bone morphogenic proteins.
Bailey et al. (2007)
13 more

17 mentions without citation

Hedgehog secretion and signal transduction in vertebrates.
Ryan et al. (2012)
16 more