› 3m1r

X-ray diffraction
2.2Å resolution

The crystal structure of formimidoylglutamase from Bacillus subtilis subsp. subtilis str. 168

Released:
DOI: 10.2210/pdb3m1r/pdb
PDB entry 3m1r coloured by chain, front view.
PDB entry 3m1r coloured by chain, side view.
PDB entry 3m1r coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Tan K, Bigelow L, Trevino D, Buck K, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
N-formimidoyl-L-glutamate + H(2)O = L-glutamate + formamide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formimidoylglutamase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 322 amino acids
Theoretical weight: 35.76 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42068 (Residues: 1-319; Coverage: 100%)
Gene names: BSU39380, EE57C, hutG
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

4 bound ligands:
Ligand CL 9 x CL
Ligand CA 12 x CA
Ligand PEG 23 x PEG
Ligand CAC 2 x CAC
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 142.038Å b: 118.98Å c: 123.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.23
Expression system: Escherichia coli

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