› 3m5t

X-ray diffraction
1.95Å resolution

Fragment tethered to Carbonic Anhydrase II H64C mutant

Released:
DOI: 10.2210/pdb3m5t/pdb
PDB entry 3m5t coloured by chain, front view.
PDB entry 3m5t coloured by chain, side view.
PDB entry 3m5t coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Schulze Wischeler J, Heine A, Klebe G

Function and Biology Details

Reactions catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Urea = cyanamide + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 29.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00918 (Residues: 1-260; Coverage: 100%)
Gene name: CA2
Sequence domains: Eukaryotic-type carbonic anhydrase
Structure domains: Alpha carbonic anhydrase

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand BFG 1 x BFG
Ligand CO3 1 x CO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21
Unit cell:
a: 42.8Å b: 41.3Å c: 72.1Å
α: 90° β: 104.3° γ: 90°
R-values:
R R work R free
0.178 0.164 0.25
Expression system: Escherichia coli

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