› 3m5u

X-ray diffraction
2.15Å resolution

Crystal Structure of Phosphoserine Aminotransferase from Campylobacter jejuni

Released:
DOI: 10.2210/pdb3m5u/pdb
PDB entry 3m5u coloured by chain, front view.
PDB entry 3m5u coloured by chain, side view.
PDB entry 3m5u coloured by chain, top view.
Source organism: Campylobacter jejuni
Entry authors: Kim Y, Gu M, Papazisi L, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192903 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoserine aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 361 amino acids
Theoretical weight: 41.37 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9PIH3 (Residues: 1-358; Coverage: 100%)
Gene names: Cj0326, serC
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand MES 2 x MES
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 54.738Å b: 105.865Å c: 70.009Å
α: 90° β: 99.22° γ: 90°
R-values:
R R work R free
0.172 0.169 0.22
Expression system: Escherichia coli BL21

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