› 3m6y

X-ray diffraction
1.45Å resolution

Structure of 4-hydroxy-2-oxoglutarate aldolase from bacillus cereus at 1.45 a resolution.

Released:
DOI: 10.2210/pdb3m6y/pdb
PDB entry 3m6y coloured by chain, front view.
PDB entry 3m6y coloured by chain, side view.
PDB entry 3m6y coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Entry authors: Filippova EV, Minasov G, Shuvalova L, Kiryukhina O, Clancy S, Joachimiak A, Anderson FW, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
(4R)-4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-Hydroxy-2-oxoglutarate aldolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 275 amino acids
Theoretical weight: 29.96 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q812M4 (Residues: 1-251; Coverage: 100%)
Gene name: BC_4844
Sequence domains: KDGP aldolase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand CL 6 x CL
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 48.679Å b: 106.187Å c: 88.205Å
α: 90° β: 90.95° γ: 90°
R-values:
R R work R free
0.153 0.152 0.18
Expression system: Escherichia coli BL21

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