› 3maz

X-ray diffraction
1.9Å resolution

Crystal Structure of the Human BRDG1/STAP-1 SH2 Domain in Complex with the NTAL pTyr136 Peptide

Released:
DOI: 10.2210/pdb3maz/pdb
PDB entry 3maz coloured by chain, front view.
PDB entry 3maz coloured by chain, side view.
PDB entry 3maz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Loops govern SH2 domain specificity by controlling access to binding pockets.
Kaneko T, Huang H, Zhao B, Li L, Liu H, Voss CK, Wu C, Schiller MR, Li SS
Sci Signal 3 ra34 (2010)
PMID: 20442417

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190461 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Signal-transducing adaptor protein 1 Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 14.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ULZ2 (Residues: 167-285; Coverage: 40%)
Gene names: BRDG1, STAP1
Sequence domains: SH2 domain
Structure domains: SH2 domain
Linker for activation of T-cells family member 2 Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.3 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9GZY6 (Residues: 133-143; Coverage: 5%)
Gene names: HSPC046, LAB, LAT2, NTAL, WBS15, WBSCR15, WBSCR5

Ligands and Environments

1 bound ligand:
Ligand MLI 1 x MLI
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P6222
Unit cell:
a: 72.64Å b: 72.64Å c: 97.84Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.212 0.236
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

The language of SH2 domain interactions defines phosphotyrosine-mediated signal transduction.
Liu et al. (2012)
9 more

6 mentions without citation

Loops govern SH2 domain specificity by controlling access to binding pockets.
Kaneko et al. (2010)
5 more