› 3me5

X-ray diffraction
1.75Å resolution

Crystal structure of putative dna cytosine methylase from shigella flexneri 2a str. 2457T

Released:
DOI: 10.2210/pdb3me5/pdb
PDB entry 3me5 coloured by chain, front view.
PDB entry 3me5 coloured by chain, side view.
PDB entry 3me5 coloured by chain, top view.
Source organism: Shigella flexneri 2a str. 2457T
Entry authors: Ramagopal UA, Malashkevich VN, Toro R, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosine-specific methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 482 amino acids
Theoretical weight: 55.27 KDa
Source organism: Shigella flexneri 2a str. 2457T
Expression system: Escherichia coli BL21(DE3)
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 149.629Å b: 51.593Å c: 63.026Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links