› 3meu

X-ray diffraction
1.28Å resolution

Crystal structure of SGF29 in complex with H3R2me2sK4me3

Released:
DOI: 10.2210/pdb3meu/pdb
PDB entry 3meu coloured by chain, front view.
PDB entry 3meu coloured by chain, side view.
PDB entry 3meu coloured by chain, top view.
Source organisms:
Primary publication:
Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation.
Bian C, Xu C, Ruan J, Lee KK, Burke TL, Tempel W, Barsyte D, Li J, Wu M, Zhou BO, Fleharty BE, Paulson A, Allali-Hassani A, Zhou JQ, Mer G, Grant PA, Workman JL, Zang J, Min J
EMBO J 30 2829-42 (2011)
PMID: 21685874

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187638 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SAGA-associated factor 29 Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 20.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q96ES7 (Residues: 115-293; Coverage: 61%)
Gene names: CCDC101, SGF29
Sequence domains: SGF29 tudor-like domain
Structure domains: SH3 type barrels.
Histone H3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 amino acids
Theoretical weight: 1.6 KDa
Source organism: Xenopus laevis
Expression system: Not provided
UniProt:
  • Canonical: Q92133 (Residues: 2-14; Coverage: 10%)
Gene names: H3l, Hist1h3f, h3, h3.2a, h3c8, h3c8.S, h3r, hist1h3g, hist1h3g.L, hist2h3, hist2h3c

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
3 modified residues:
Ligand MSE 4 x MSE
Ligand 2MR 2 x 2MR
Ligand M3L 2 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 50.14Å b: 65.483Å c: 106.004Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.228
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

52 review citations

Perceiving the epigenetic landscape through histone readers.
Musselman et al. (2012)
51 more

3 mentions without citation

Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation.
Bian et al. (2011)
2 more