› 3mgd

X-ray diffraction
1.9Å resolution

Crystal Structure of predicted acetyltransferase with acetyl-CoA from Clostridium acetobutylicum at the resolution 1.9A, Northeast Structural Genomics Consortium Target CaR165

Released:
DOI: 10.2210/pdb3mgd/pdb
PDB entry 3mgd coloured by chain, front view.
PDB entry 3mgd coloured by chain, side view.
PDB entry 3mgd coloured by chain, top view.
Source organism: Clostridium acetobutylicum
Entry authors: Kuzin A, Chen Y, Seetharaman J, Sahdev S, Xiao R, Ciccosanti C, Zhao L, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyltransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 157 amino acids
Theoretical weight: 18.52 KDa
Source organism: Clostridium acetobutylicum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97MN4 (Residues: 3-149; Coverage: 99%)
Gene name: CA_C0160
Sequence domains: Acetyltransferase (GNAT) domain
Structure domains: Aminopeptidase

Ligands and Environments

1 bound ligand:
Ligand ACO 2 x ACO
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: C2
Unit cell:
a: 57.933Å b: 56.341Å c: 99.721Å
α: 90° β: 98.86° γ: 90°
R-values:
R R work R free
0.182 0.18 0.228
Expression system: Escherichia coli BL21(DE3)

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