› 3mjs

X-ray diffraction
1.4Å resolution

Structure of A-type Ketoreductases from Modular Polyketide Synthase

Released:
DOI: 10.2210/pdb3mjs/pdb
PDB entry 3mjs coloured by chain, front view.
PDB entry 3mjs coloured by chain, side view.
PDB entry 3mjs coloured by chain, top view.
Source organism: Streptomyces nodosus
Primary publication:
Structural and functional analysis of A-type ketoreductases from the amphotericin modular polyketide synthase.
Zheng J, Taylor CA, Piasecki SK, Keatinge-Clay AT
Structure 18 913-22 (2010)
PMID: 20696392

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188105 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AmphB Chains: A, B
Molecule details ›
Chains: A, B
Length: 496 amino acids
Theoretical weight: 51.42 KDa
Source organism: Streptomyces nodosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93NW7 (Residues: 2529-3003; Coverage: 15%)
Gene name: amphB
Sequence domains: KR domain
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NDP 2 x NDP
3 bound ligands:
Ligand LMR 1 x LMR
Ligand MLT 3 x MLT
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 61.439Å b: 63.71Å c: 71.824Å
α: 72.77° β: 67.09° γ: 89.79°
R-values:
R R work R free
0.2 0.199 0.226
Expression system: Escherichia coli

Quick links

Citations

13 review citations

The structures of type I polyketide synthases.
Keatinge-Clay AT. (2012)
12 more

5 mentions without citation

Stereocontrol within polyketide assembly lines.
Keatinge-Clay AT. (2016)
4 more