› 3mls

X-ray diffraction
2.5Å resolution

Crystal structure of anti-HIV-1 V3 mAb 2557 Fab in complex with a HIV-1 gp120 V3 mimotope

Released:
DOI: 10.2210/pdb3mls/pdb
PDB entry 3mls coloured by chain, front view.
PDB entry 3mls coloured by chain, side view.
PDB entry 3mls coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conserved structural elements in the V3 crown of HIV-1 gp120.
Jiang X, Burke V, Totrov M, Williams C, Cardozo T, Gorny MK, Zolla-Pazner S, Kong XP
Nat Struct Mol Biol 17 955-61 (2010)
PMID: 20622876

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211636 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Human monoclonal anti-HIV-1 gp120 V3 antibody 2557 Fab light chain Chains: L, M, N, O
Molecule details ›
Chains: L, M, N, O
Length: 219 amino acids
Theoretical weight: 23.33 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
Human monoclonal anti-HIV-1 gp120 V3 antibody 2557 Fab heavy chain Chains: H, I, J, K
Molecule details ›
Chains: H, I, J, K
Length: 226 amino acids
Theoretical weight: 24.19 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
Rationally designed V3 mimotope Chains: P, Q, R, S
Molecule details ›
Chains: P, Q, R, S
Length: 20 amino acids
Theoretical weight: 2.08 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2
Unit cell:
a: 168.746Å b: 43.051Å c: 274.341Å
α: 90° β: 94.2° γ: 90°
R-values:
R R work R free
0.216 0.216 0.274

Quick links

Citations

2 review citations

Requirements for empirical immunogenicity trials, rather than structure-based design, for developing an effective HIV vaccine.
Van Regenmortel MH. (2012)
1 more

6 mentions without citation

Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env.
Kwon et al. (2015)
5 more