› 3mlu

X-ray diffraction
2.77Å resolution

Crystal structure of anti-HIV-1 V3 Fab 2557 in complex with a ZAM18 V3 peptide

Released:
DOI: 10.2210/pdb3mlu/pdb
PDB entry 3mlu coloured by chain, front view.
PDB entry 3mlu coloured by chain, side view.
PDB entry 3mlu coloured by chain, top view.
Source organisms:
Primary publication:
Conserved structural elements in the V3 crown of HIV-1 gp120.
Jiang X, Burke V, Totrov M, Williams C, Cardozo T, Gorny MK, Zolla-Pazner S, Kong XP
Nat Struct Mol Biol 17 955-61 (2010)
PMID: 20622876

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207283 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Human monoclonal anti-HIV-1 gp120 V3 antibody 2557 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 23.27 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
Human monoclonal anti-HIV-1 gp120 V3 antibody 2557 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 226 amino acids
Theoretical weight: 24.19 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
Human immunodeficiency virus 1 envelope glycoprotein Gp120 domain-containing protein Chain: P
Molecule details ›
Chain: P
Length: 23 amino acids
Theoretical weight: 2.39 KDa
Source organism: Human immunodeficiency virus 1
UniProt:
  • Canonical: A0A0K0KAD3 (Residues: 71-93; Coverage: 19%)
Gene name: env

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P1
Unit cell:
a: 42.452Å b: 43.167Å c: 57.824Å
α: 89.15° β: 86.25° γ: 85.19°
R-values:
R R work R free
0.202 0.196 0.31

Quick links

Citations

2 review citations

Requirements for empirical immunogenicity trials, rather than structure-based design, for developing an effective HIV vaccine.
Van Regenmortel MH. (2012)
1 more

1 mention without citation

Isolation of monoclonal antibodies with predetermined conformational epitope specificity.
Sholukh et al. (2012)