› 3mlx

X-ray diffraction
1.9Å resolution

Crystal structure of anti-HIV-1 V3 Fab 3074 in complex with an MN V3 peptide

Released:
DOI: 10.2210/pdb3mlx/pdb
PDB entry 3mlx coloured by chain, front view.
PDB entry 3mlx coloured by chain, side view.
PDB entry 3mlx coloured by chain, top view.
Source organisms:
Primary publication:
Conserved structural elements in the V3 crown of HIV-1 gp120.
Jiang X, Burke V, Totrov M, Williams C, Cardozo T, Gorny MK, Zolla-Pazner S, Kong XP
Nat Struct Mol Biol 17 955-61 (2010)
PMID: 20622876

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209257 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Human monoclonal anti-HIV-1 gp120 V3 antibody 3074 Fab light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 211 amino acids
Theoretical weight: 22.3 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
Human monoclonal anti-HIV-1 gp120 V3 antibody 3074 Fab heavy chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 228 amino acids
Theoretical weight: 24.28 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
Surface protein gp120 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 23 amino acids
Theoretical weight: 2.71 KDa
Source organism: HIV-1 M:B_MN
UniProt:
  • Canonical: P05877 (Residues: 306-328; Coverage: 3%)
Gene name: env

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21
Unit cell:
a: 59.87Å b: 128.796Å c: 60.189Å
α: 90° β: 92.66° γ: 90°
R-values:
R R work R free
0.192 0.192 0.228

Quick links

Citations

2 review citations

Requirements for empirical immunogenicity trials, rather than structure-based design, for developing an effective HIV vaccine.
Van Regenmortel MH. (2012)
1 more

7 mentions without citation

Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env.
Kwon et al. (2015)
6 more