› 3mo4

X-ray diffraction
1.9Å resolution

The crystal structure of an alpha-(1-3,4)-fucosidase from Bifidobacterium longum subsp. infantis ATCC 15697

Released:
DOI: 10.2210/pdb3mo4/pdb
PDB entry 3mo4 coloured by chain, front view.
PDB entry 3mo4 coloured by chain, side view.
PDB entry 3mo4 coloured by chain, top view.
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Primary publication:
Bifidobacterium longum subsp. infantis ATCC 15697 α-fucosidases are active on fucosylated human milk oligosaccharides.
Sela DA, Garrido D, Lerno L, Wu S, Tan K, Eom HJ, Joachimiak A, Lebrilla CB, Mills DA
Appl Environ Microbiol 78 795-803 (2012)
PMID: 22138995

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
F5/8 type C domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 480 amino acids
Theoretical weight: 53.75 KDa
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B7GNN8 (Residues: 1-478; Coverage: 100%)
Gene name: Blon_2336
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand TYR 1 x TYR
Ligand FMT 1 x FMT
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 82.61Å b: 106.094Å c: 122.383Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.205
Expression system: Escherichia coli BL21

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Citations

37 review citations

Mucin glycan foraging in the human gut microbiome.
Tailford et al. (2015)
36 more

7 mentions without citation

Structure and evolution of the bifidobacterial carbohydrate metabolism proteins and enzymes.
Fushinobu et al. (2021)
6 more