› 3mqm

X-ray diffraction
2.54Å resolution

Crystal Structure of the Bromodomain of human ASH1L

Released:
DOI: 10.2210/pdb3mqm/pdb
PDB entry 3mqm coloured by chain, front view.
PDB entry 3mqm coloured by chain, side view.
PDB entry 3mqm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Histone recognition and large-scale structural analysis of the human bromodomain family.
Filippakopoulos P, Picaud S, Mangos M, Keates T, Lambert JP, Barsyte-Lovejoy D, Felletar I, Volkmer R, Müller S, Pawson T, Gingras AC, Arrowsmith CH, Knapp S
Cell 149 214-31 (2012)
PMID: 22464331

Function and Biology Details

Reactions catalysed: 
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(36) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(36)
S-adenosyl-L-methionine + a [histone H3]-L-lysine(9) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(9)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-192538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-lysine N-methyltransferase ASH1L Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 14.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR48 (Residues: 2438-2561; Coverage: 4%)
Gene names: ASH1L, KIAA1420, KMT2H
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P65
Unit cell:
a: 69.59Å b: 69.59Å c: 151.34Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.204 0.259
Expression system: Escherichia coli

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Citations

244 review citations

Modification of enhancer chromatin: what, how, and why?
Calo et al. (2013)
243 more

5 mentions without citation

Modulation of epigenetic targets for anticancer therapy: clinicopathological relevance, structural data and drug discovery perspectives.
Andreoli et al. (2013)
4 more