› 3mqr

X-ray diffraction
1.8Å resolution

Crystal Structure of the USP7:HdmX(AHSS) complex

Released:
DOI: 10.2210/pdb3mqr/pdb
PDB entry 3mqr coloured by chain, front view.
PDB entry 3mqr coloured by chain, side view.
PDB entry 3mqr coloured by chain, top view.
Source organism: Homo sapiens
Entry author: Saridakis V

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127328 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin carboxyl-terminal hydrolase 7 Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 18.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93009 (Residues: 54-205; Coverage: 14%)
Gene names: HAUSP, USP7
Sequence domains: MATH domain
Structure domains: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
Protein Mdm4 Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O15151 (Residues: 395-404; Coverage: 2%)
Gene names: MDM4, MDMX

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41
Unit cell:
a: 69.76Å b: 69.76Å c: 45.493Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.228
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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