› 3mr0

X-ray diffraction
1.49Å resolution

Crystal Structure of Sensory Box Histidine Kinase/Response Regulator from Burkholderia thailandensis E264

Released:
DOI: 10.2210/pdb3mr0/pdb
PDB entry 3mr0 coloured by chain, front view.
PDB entry 3mr0 coloured by chain, side view.
PDB entry 3mr0 coloured by chain, top view.
Source organism: Burkholderia thailandensis E264
Entry authors: Kim Y, Tesar C, Buck K, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensory box histidine kinase/response regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 142 amino acids
Theoretical weight: 16.73 KDa
Source organism: Burkholderia thailandensis E264
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q2T6J3 (Residues: 106-245; Coverage: 30%)
Gene name: BTH_II1009
Sequence domains: PAS fold
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand SO4 2 x SO4
Ligand PG5 6 x PG5
Ligand GOL 2 x GOL
Ligand PEG 4 x PEG
Ligand EDO 2 x EDO
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 59.265Å b: 35.08Å c: 71.348Å
α: 90° β: 90.06° γ: 90°
R-values:
R R work R free
0.165 0.164 0.192
Expression system: Escherichia coli BL21

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