› 3mtc

X-ray diffraction
2.4Å resolution

Crystal Structure of INPP5B in complex with phosphatidylinositol 4-phosphate

Released:
DOI: 10.2210/pdb3mtc/pdb
PDB entry 3mtc coloured by chain, front view.
PDB entry 3mtc coloured by chain, side view.
PDB entry 3mtc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for phosphoinositide substrate recognition, catalysis, and membrane interactions in human inositol polyphosphate 5-phosphatases.
Trésaugues L, Silvander C, Flodin S, Welin M, Nyman T, Gräslund S, Hammarström M, Berglund H, Nordlund P
Structure 22 744-55 (2014)
PMID: 24704254

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-152190 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II inositol 1,4,5-trisphosphate 5-phosphatase Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 36.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32019 (Residues: 339-643; Coverage: 31%)
Gene names: INPP5B, OCRL2
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

5 bound ligands:
Ligand GOL 2 x GOL
Ligand MG 1 x MG
Ligand PIF 1 x PIF
Ligand SO4 1 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P213
Unit cell:
a: 133.761Å b: 133.761Å c: 133.761Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The extended human PTPome: a growing tyrosine phosphatase family.
Alonso et al. (2016)
2 more

11 mentions without citation

Inositol 5-phosphatases: insights from the Lowe syndrome protein OCRL.
Pirruccello et al. (2012)
10 more