› 3mtj

X-ray diffraction
2.15Å resolution

The Crystal Structure of a Homoserine Dehydrogenase from Thiobacillus denitrificans to 2.15A

Released:
DOI: 10.2210/pdb3mtj/pdb
PDB entry 3mtj coloured by chain, front view.
PDB entry 3mtj coloured by chain, side view.
PDB entry 3mtj coloured by chain, top view.
Source organism: Thiobacillus denitrificans ATCC 25259
Entry authors: Stein AJ, Cui H, Chin S, Savchenko A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
homoserine dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 444 amino acids
Theoretical weight: 48.39 KDa
Source organism: Thiobacillus denitrificans ATCC 25259
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3SKI5 (Residues: 1-437; Coverage: 100%)
Gene name: Tbd_0843
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P42212
Unit cell:
a: 67.613Å b: 67.613Å c: 224.279Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.256
Expression system: Escherichia coli BL21(DE3)

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