› 3mvk

X-ray diffraction
1.65Å resolution

The Crystal Structure of FucU from Bifidobacterium longum to 1.65A

Released:
DOI: 10.2210/pdb3mvk/pdb
PDB entry 3mvk coloured by chain, front view.
PDB entry 3mvk coloured by chain, side view.
PDB entry 3mvk coloured by chain, top view.
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Entry authors: Stein AJ, Xu X, Cui H, Savchenko A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reactions catalysed: 
Alpha-L-fucose = beta-L-fucose
Beta-D-ribopyranose = beta-D-ribofuranose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo decamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110468 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RbsD or FucU transport Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 148 amino acids
Theoretical weight: 16.15 KDa
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B7GNK7 (Residues: 1-148; Coverage: 100%)
Gene name: Blon_2305
Sequence domains: RbsD / FucU transport protein family
Structure domains: RbsD-like domain

Ligands and Environments

3 bound ligands:
Ligand NA 15 x NA
Ligand PGE 11 x PGE
Ligand GOL 6 x GOL
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 66.047Å b: 83.859Å c: 144.813Å
α: 90° β: 95.11° γ: 90°
R-values:
R R work R free
0.183 0.182 0.205
Expression system: Escherichia coli BL21(DE3)

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