› 3mwc

X-ray diffraction
1.8Å resolution

Crystal structure of probable o-succinylbenzoic acid synthetase from kosmotoga olearia

Released:
DOI: 10.2210/pdb3mwc/pdb
PDB entry 3mwc coloured by chain, front view.
PDB entry 3mwc coloured by chain, side view.
PDB entry 3mwc coloured by chain, top view.
Source organism: Kosmotoga olearia TBF 19.5.1
Entry authors: Patskovsky Y, Toro R, Dickey M, Sauder JM, Gerlt J, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111471 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
o-succinylbenzoate synthase Chain: A
Molecule details ›
Chain: A
Length: 400 amino acids
Theoretical weight: 45.17 KDa
Source organism: Kosmotoga olearia TBF 19.5.1
Expression system: Escherichia coli
UniProt:
  • Canonical: C5CFI0 (Residues: 3-391; Coverage: 100%)
Gene name: Kole_0682
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 138.171Å b: 53.233Å c: 59.386Å
α: 90° β: 113.4° γ: 90°
R-values:
R R work R free
0.21 0.209 0.262
Expression system: Escherichia coli

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