› 3mxq

X-ray diffraction
2.78Å resolution

Crystal structure of sensory box sensor histidine kinase from Vibrio cholerae

Released:
DOI: 10.2210/pdb3mxq/pdb
PDB entry 3mxq coloured by chain, front view.
PDB entry 3mxq coloured by chain, side view.
PDB entry 3mxq coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Entry authors: Chang C, Wu R, Freeman L, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192079 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 152 amino acids
Theoretical weight: 17.85 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KT21 (Residues: 1-136; Coverage: 31%)
Gene name: VC_1084
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 58.237Å b: 102.202Å c: 124.969Å
α: 90° β: 100.12° γ: 90°
R-values:
R R work R free
0.227 0.225 0.257
Expression system: Escherichia coli BL21(DE3)

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