› 3mxv

X-ray diffraction
1.9Å resolution

Crystal structure of fab fragment of anti-Shh 5E1 chimera

Released:
DOI: 10.2210/pdb3mxv/pdb
PDB entry 3mxv coloured by chain, front view.
PDB entry 3mxv coloured by chain, side view.
PDB entry 3mxv coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Hedgehog pathway antagonist 5E1 binds hedgehog at the pseudo-active site.
Maun HR, Wen X, Lingel A, de Sauvage FJ, Lazarus RA, Scales SJ, Hymowitz SG
J Biol Chem 285 26570-80 (2010)
PMID: 20504762

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213329 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
fab fragment of anti-Shh 5E1 mouse/human chimera, light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
fab fragment of anti-Shh 5E1 mouse/human chimera, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 24.12 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: R3
Unit cell:
a: 110.287Å b: 110.287Å c: 122.509Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.179 0.224
Expression system: Escherichia coli

Quick links

Citations

35 review citations

Unraveling the therapeutic potential of the Hedgehog pathway in cancer.
Amakye et al. (2013)
34 more

5 mentions without citation

Updates to the Integrated Protein-Protein Interaction Benchmarks: Docking Benchmark Version 5 and Affinity Benchmark Version 2.
Vreven et al. (2015)
4 more