› 3n04

X-ray diffraction
2.02Å resolution

THE CRYSTAL STRUCTURE OF THE alpha-Glucosidase (FAMILY 31) FROM RUMINOCOCCUS OBEUM ATCC 29174

Released:
DOI: 10.2210/pdb3n04/pdb
PDB entry 3n04 coloured by chain, front view.
PDB entry 3n04 coloured by chain, side view.
PDB entry 3n04 coloured by chain, top view.
Source organism: Blautia obeum ATCC 29174
Primary publication:
Novel α-glucosidase from human gut microbiome: substrate specificities and their switch.
Tan K, Tesar C, Wilton R, Keigher L, Babnigg G, Joachimiak A
FASEB J 24 3939-49 (2010)
PMID: 20581222

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase, family 31 Chains: A, B
Molecule details ›
Chains: A, B
Length: 666 amino acids
Theoretical weight: 78.72 KDa
Source organism: Blautia obeum ATCC 29174
Expression system: Escherichia coli
UniProt:
  • Canonical: A5ZY13 (Residues: 1-663; Coverage: 100%)
Gene name: RUMOBE_03919
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
1 modified residue:
Ligand MSE 54 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 68.861Å b: 124.97Å c: 88.601Å
α: 90° β: 107.74° γ: 90°
R-values:
R R work R free
0.17 0.168 0.207
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Intestinal SGLT1 in metabolic health and disease.
Lehmann et al. (2016)
3 more

4 mentions without citation

Heterocyclic compounds as a magic bullet for diabetes mellitus: a review.
Farwa et al. (2022)
3 more